1) 女性（雌性）生殖调控机制（基础研究）

2) 女性生育力异常的病理机制（基础研究）

2) 女性生育力异常的干预技术（应用基础）

研究组围绕女性生殖调节机制的研究，聚焦于女性生殖调控机制和女性生育力异常的病理机制研究，对标临床的多囊卵巢综合征、卵巢储备功能低下、卵巢早衰、早期自然流产，反复植入失败、子痫前期以及宫内生长受限等生殖相关疾病，并结合类器官技术，研究女性生殖相关疾病的病因和病理机制，旨在寻找生殖相关疾病靶点和标志分子以及干预技术产品的开发，并建立临床诊断和治疗的新技术。

发表文章：研究组近五年共发表文章36篇，其中发表一区杂志7篇，2区杂志12篇，代表性论文：

1. Qian Yang, Yeling Ma, Yanlei Liu, Xuan Shao, Wentong Jia, Xin Yu, Yu-Xia Li, Long Yang, Wenwen Gu, Haibin Wang, Jian Wang, Yan-Ling Wang. MNSFβ regulates placental development by conjugating IGF2BP2 to enhance trophoblast cell invasiveness. Cell Prolif. 2021 Dec;54(12):e13145. doi: 10.1111/cpr.13145.

2. Shu-Han Yang#, Long Yang#, Yan Shi, Hao-Ran Xu, Jie Gan, Jia-Xin Shi, Yu Zhang, Shi-Long Shen, Jian Wang∗, Xuan Zhang∗. GDF15 promotes trophoblast invasion and pregnancy success via the BMPR1A/BMPR2/p-SMAD1 pathway: Implications for recurrent miscarriage. Life Sci, 2025 Mar 27:371:123586. doi: 10.1016/j.lfs.2025.123586.

3. Xing-Xing Zhen, Long Yang, Yan Gu, Qian Yang, Wen-Wen Gu, Ya-Ping He, Yan-Ling Wang, Jian Wang. MNSFβ Regulates TNFα Production by Interacting with RC3H1 in Human Macrophages, and Dysfunction of MNSFβ in Decidual Macrophages Is Associated With Recurrent Pregnancy Loss. Front Immunol. 2021 Sep 13:12:691908. doi: 10.3389/fimmu.2021.691908. eCollection 2021.

4. Long Yang, Hao-Ran Xu, Xuan Zhang, Yan Shi, Jia-Xin Shi, Qian-Qian Chen, Xiao-Rong Shen, Ya-Ping He, Jia-Nan Tang, Wen-Wen Gu, Jian Wang. Increased miR-3074-5p expression promotes M1 polarization and pyroptosis of macrophages via ERα/NLRP3 pathway and induces adverse pregnancy outcomes in mice. Cell Death Discov. 2024 Apr 10;10(1):171. doi: 10.1038/s41420-024-01941-4.

5. Yan Gu, Ning Song, Wen-Wen Gu, Linghan Chen, Xuan Zhang, Jian Wang. Down-regulation of miR-486-5p expression inhibits the proliferation and invasion of human extravillous trophoblasts, and decreased peripheral plasma miR-486-5p level is associated with human early pregnancy loss. Acta Biochim Biophys Sin (Shanghai). 2023 Nov 25;55(11):1847-1850. doi: 10.3724/abbs.2023239.

6. Jiali Luo, Jian Zhang, Yu Zhang, Meihui Li, Lin Yu, Di Song, Zhaogui Sun. Genetic loss of Uchl1 leads to female infertility by affecting oocyte quality and follicular development. Mol Cell Endocrinol. 2025 Feb 1:597:112440. doi: 10.1016/j.mce.2024.112440.

7. Han-Yu Jiang, Wen-Wen Gu, Jie Gan, Qian Yang, Yan Shi, Wen-Bo Lian, Hao-Ran Xu, Shu-Han Yang, Long Yang, Xuan Zhang, Jian Wang. MNSFβ promotes LPS-induced TNFα expression by increasing the localization of RC3H1 to stress granules, and the interfering peptide HEPN2 reduces TNFα production by disrupting the MNSFβ-RC3H1 interaction in macrophages. Int Immunopharmacol. 2024 Dec 5;142(Pt A):113053. doi: 10.1016/j.intimp.2024.113053.

8. Jia-Xin Shi, Long Yang , Jie Gan, Wen-Wen Gu, Yan Gu, Yan Shi, Han-Yu Jiang, Hao-Ran Xu, Shu-Han Yang, Xuan Zhang, Jian Wang. MiR-3074-5p Regulates Trophoblasts Function via EIF2S1/GDF15 Pathway in Recurrent Miscarriage. Reprod Sci. . 2024 May;31(5):1290-1302. doi: 10.1007/s43032-023-01436-0.

9. Shu-Han Yang#, Jie Gan#, Hao-Ran Xu, Jia-Xin Shi, Jian Wang∗, Xuan Zhang∗. The BMP Signaling Pathway: Bridging Maternal-Fetal Crosstalk in Early Pregnancy Review Reprod Sci, 2025 Jan 16. doi: 10.1007/s43032-024-01777-4.

10. Nan Meng, Xinyue Wang, Yan Shi, Yanyan Mao, Qian Yang, Baohui Ju, Qianxi Zhu, Tingting Zhang, Yan Gu*, and Xuan Zhang*. miR-3074-5p/CLN8 pathway regulates decidualization in recurrent miscarriage. Reproduction. May 2021, Volume 162, Issue 1. doi.org/10.1530/REP-21-0032

11. Xinyi Li,# Jiaxin Shi,# Weijie Zhao, Xixi Huang, Liyuan Cui1, Lu Liu, Xueling Jin, Djin Li, Xuan Zhang* and Meirong Du*. WNT16 from decidual stromal cells regulates HTR8/SVneo trophoblastic cell function via AKT/beta-catenin pathway. Reproduction. 2022 Mar 10;163(5):241-250. doi: 10.1530/REP-21-0282.

12. Tianyu Wu, Jie Dong, Jing Fu, Yanping Kuang, Biaobang Chen, Hao Gu, Yuxi Luo, Ruihuan Gu, Meiling Zhang, Wen Li, Xi Dong, Xiaoxi Sun, Qing Sang, Lei Wang. Mechanisms of minor pole–mediated spindle bipolarization in human oocytes. Science.2024 Aug 23;385(6711):eado1022. doi: 10.1126/science.ado1022.

13. Zhiqi Pan, Weijie Wang, Ling Wu, Zhongyuan Yao, Wenjing Wang, Yao Chen, Hao Gu, Jie Dong, Jian Mu, Zhihua Zhang, Jing Fu, Qiaoli Li, Lei Wang, Xiaoxi Sun, Yanping Kuang, Qing Sang, Biaobang Chen. Bi-allelic missense variants in MEI4 cause preimplantation embryonic arrest and female infertility. Hum Genet. 2024 Oct;143(9-10):1049-1060. doi: 10.1007/s00439-023-02633-2.

14. Hao-Ran Xu, Long Yang, Yan Gu, Yan Shi, Shu-Han Yang, Jie Gan, Wen-Wen Gu, Xuan Zhang, Jian Wang. Identification of potential hub genes associated with recurrent miscarriage through combined transcriptomic and proteomic analysis. Biomol Biomed. 2024 Oct 20. doi: 10.17305/bb.2024.11158.

15. Wenjing Wang, Yoichi Miyamoto, Biaobang Chen, Juanzi Shi, Feiyang Diao, Wei Zheng, Qun Li, Lan Yu, Lin Li, Yao Xu, Ling Wu, Xiaoyan Mao, Jing Fu, Bin Li, Zheng Yan, Rong Shi, Xia Xue, Jian Mu, Zhihua Zhang, Tianyu Wu, Lin Zhao, Weijie Wang, Zhou Zhou, Jie Dong, Qiaoli Li, Li Jin, Lin He, Xiaoxi Sun, Ge Lin, Yanping Kuang, Lei Wang, Qing Sang. Karyopherin α deficiency contributes to human preimplantation embryo arrest. J Clin Invest. 2023 Jan 17;133(2):e159951. doi: 10.1172/JCI159951.

16. Huizhen Fan, Zhou Zhou, Wei Zheng, Yichun Guan, Qingxia Meng, Wenjing Wang, Jie Dong, Liuxia Wan, Jiawei Zhu, Yang Zeng, Ruyi Liu, Hao Gu, Ge Lin, Biaobang Chen, Qing Sang, Lei Wang. Homozygous variants in CDC23 cause female infertility characterized by oocyte maturation defects. Hum Genet. 2023 Nov;142(11):1621-1631. doi: 10.1007/s00439-023-02606-5. Epub 2023 Sep 28.

17. Ronggui Qu, Zhihua Zhang, Ling Wu, Qun Li, Jian Mu, Lin Zhao, Zheng Yan, Wenjing Wang, Yang Zeng, Ruyi Liu, Jie Dong, Qiaoli Li, Xiaoxi Sun, Lei Wang, Qing Sang, Biaobang Chen, Yanping Kuang. ADGB variants cause asthenozoospermia and male infertility. Hum Genet. 2023 Jun;142(6):735-748. doi: 10.1007/s00439-023-02546-0. 

18. Zhou Zhou, Huizhen Fan, Rong Shi, Yang Zeng, Ruyi Liu, Hao Gu, Qiaoli Li, Qing Sang, Lei Wang, Juanzi Shi, Biaobang Chen. A novel homozygous variant in ZFP36L2 cause female infertility due to oocyte maturation defect. Clin Genet. 2023 Oct;104(4):461-465. doi: 10.1111/cge.14362.

19. Jie Gan # 1, Long Yang # , Shu-Han Yang, Wen-Wen Gu, Yan Gu, Yan Shi, Jia-Xin Shi, Hao-Ran Xu, Ya-Wei Xin, Xuan Zhang∗, Jian Wang∗. FXYD1 was identified as a hub gene in recurrent miscarriage and involved in decidualization via regulating Na/K-ATPase activity. J Assist Reprod Genet. 2024 Dec 27. doi: 10.1007/s10815-024-03363-8.

20. Hai-Ping Tang, Ya-Ping He, Jian Wang, Jian-Min Zhan, Wen-Bo Lian, Feng Xue, Li Wang, Yijie Li, Ailian Zhang, Fuchun Zhang, Chen Xu, Jinyao Li, Wan-Xiang Xu. Epitope delimitation: A new method for defining epitopes of human IgG-reactive antigenic peptides based on rabbit-recognized epitope motifs. J Med Virol. 2024 Jan;96(1):e29388. doi: 10.1002/jmv.29388.

专利申请：近五年已授权2项国家发明专利，申请国家发明专利7项：

1. 国家发明专利：一种体外卵母细胞培养体系及其应用，CN 107841484 B，2024年已授权；

2. 国家发明专利：一种MNSFβ/RC3H1 PPI干扰多肽的抗炎治疗应用，CN 116459344 B，2025已授权；

3. 国家发明专利：免疫抑制因子MNSFbeta在蜕膜巨噬细胞中的作用及在反复流产中的应用， 202110139640.0；

4. 国家发明专利：miR-146a-5p 和/或 miR-486-5p 在诊断先兆流产中的应用，2023105530554；

5. 国家发明专利：一种 MNSFβ/RC3H1 PPI 干扰多肽的抗炎治疗应用，2023104751403；

6. 国家发明专利：一种卵巢颗粒细胞立体培养方法及其应用，2024100150093；

7. 国家发明专利：圣草酚在制备预防和/或治疗复发性自然流产的药物中的应用，2024111866497；

8. 国家发明专利：免疫抑制因子MNSFbeta在蜕膜巨噬细胞中的作用及在反复流产中的应用，202110139640.0；

9. 国家发明专利：GDF15 和 ITIH3 在孕早期自然流产预测中的应用，国家发明专利，2020115919404